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The growth of newborn rat tibiae in a continuousflow organ culture system

✍ Scribed by Schwartz, P. L. ;Wettenhall, R. E. H. ;Bornstein, J.

Publisher: John Wiley and Sons
Year: 1968
Tongue: English
Weight: 927 KB
Volume: 168
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1401680412

No coin nor oath required. For personal study only.

✦ Synopsis

A technique for continuous-flow organ culture of newborn rat tibiae in a chemically defined medium is described. The growth characteristics of such bones during three days of culture, in terms of length, weight, hydroxyproline content, galactosamine content, and calcium content, are compared with corresponding growth in vivo and are seen to be reduced, especially beyond the first day of culture. Net synthesis of hydroxyproline and galactosamine during culture is demonstrated.

It is shown that cultured bones are capable of incorporating labelled proline into hydroxyproline and labelled sulphate into bound form linearly for 72 hours, indicating continuing matrix synthesis in vitro.

The implications of these findings are discussed.

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