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The genetic fine structure of nonsense suppressors inSchizosaccharomyces pombe

✍ Scribed by P. Munz; K. Dorsch-Häsler; U. Leupold

Publisher
Springer-Verlag
Year
1983
Tongue
English
Weight
887 KB
Volume
7
Category
Article
ISSN
0172-8083

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✦ Synopsis

Meiotic fine-structure maps of two efficient UGA suppressors of Schizosaccharomyces pombe which are known (sup8-e) or inferred (suplO-e) to code for two leucine tRNAs carrying the mutant anticodon U'CA (Kohli et al. 1979(Kohli et al. , 1980a, b;, b;Wetzel et al. 1979;Mao et al. 1981) are presented. In both cases, the recombination frequencies given by the primary site of the anticodon mutation fitwell into the map defined by the sites of a number of inactivating secondary mutations. This contrasts the corresponding situation found in the serine tRNA genes sup3 and sup9 where the anticodon site exhibits a specific marker effect which strongly increases recombination frequencies in crosses with all revertant sites, due to a decrease in the efficiency of excision repair of base-pair mismatches whenever the anticodon site is included in hybrid-DNA (Hofer et al. 1979; Munz and Leupold 1979; Thurianx et al. 1980). A pronounced specific marker effect which leads to a severalfold increase of the recombination frequencies over those expected is observed, however, at one of the secondary inactivating sites mapping in the leucine tRNA gene supS.

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