The generation of nanocolloidal wear from stainless steel and titanium alloy and its toxic effects in a monocyte cell culture model
✍ Scribed by L.E. Podleska; M. Weuster; R. Buescher; B. Hussmann; D. Nast-Kolb; G. Taeger
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 305 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0933-5137
No coin nor oath required. For personal study only.
✦ Synopsis
This study addresses the aspect of submicron-sized / nanocolloidal wear comparing stainless steel (316L) and titanium alloy (TiAl6V4) for their toxic and inflammatory potentials. Wear was generated in a tribometer using the disc-on-pin-method with pin and disc submerged in a sterile cell culture medium setting. The wear medium was separated according to Stokes' law into a fraction smaller 200 nm (nanocolloids) and a fraction greater 200 nm (particles). Vitality and inflammatory potential was measured in a cell culture model employing murine macrophages (J774). Cells were incubated with increasing concentrations (12.5, 25 and 50vol%) of either wear medium (particles and nanocolloids) from 316L and TiAl6V4. Vitality was measured by MTT assay and inflammatory reactions were quantified by TNF-a ELISA.
Nanocolloids from stainless steel and titanium induced strong, dose dependant toxic effects in the MTT assay while particles did not affect vitality in a dose dependant manner. The inflammatory response remained unaltered in all four groups. We conclude that interactions between soluble metallic wear and proteins forming nanocolloidal wear should be considered when conduction experiments addressing the aspect of biocompatibility in metallic implant materials.