The rplY gene of Escherichia coli K12 encoding ribosomal protein L25 was cloned from the ordered clone bank and located at coordinate 2,291 kb on the physical map of E. coli. Determination of the nucleotide sequence indicated that the coding region contains 285 nucleotide pairs including a translati
The gene for ribosomal protein L25 (rplY) maps at 47.3 min near nalA in Escherichia coli K-12
โ Scribed by Schnier, Joachim ;Isono, Katsumi
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 886 KB
- Volume
- 176
- Category
- Article
- ISSN
- 0026-8925
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โฆ Synopsis
A temperature sensitive mutant, termed JE1306, derived from Escherichia coli strain PA3092 was found to have an alteration in the ribosomal protein L25. Crosses with various Hfr strains and transductions with P1 kc phage have revealed that the mutation maps at 47.3 min between nalA and fpk, in a region where no ribosomal protein gene has so far been located. The gene affected by this mutation is most probably the structural gene for protein L25 (rplY), because a strain heteromerozygous for the region shows both wild type and mutant forms of protein L25.
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A mutant of E. coli selected for temperature-sensitive growth on rich medium harbored an altered ribosomal protein S6 (Isono et al., 1976). This mutant was found to possess at least two mutations, one being responsible for the temperature-sensitivity and the other for the S6 alteration. Crosses with