The gene for cadmium metallothionein from a cadmium-resistant yeast appears to be identical toCUP 1in a copper-resistant strain
✍ Scribed by Hiroshi Tohoyama; Toshifumi Tomoyasu; Masahiro Inouhe; Masanori Joho; Tetsuo Murayama
- Publisher
- Springer-Verlag
- Year
- 1992
- Tongue
- English
- Weight
- 834 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0172-8083
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✦ Synopsis
A cadmium-resistant strain of Saccharomyces cerevisiae produces a cadmium metallothionein with the same characteristics as the copper metallothionein that is encoded by CUP 1 in a copper-resistant strain. The structural gene for metallothionein from the cadmium-resistant strain resembles CUP1 in terms of the fragmentation patterns generated by restriction enzymes. Furthermore, the gene may be amplified as 2.0 kb repeating units in both the cadmium-resistant and the copperresistant strains. However, transformants with a plasmid that carried the metallothionein gene from the cadmiumresistant strain were resistant to copper but not to cadmium. It appears that the same metallothionein gene, CUP1, is amplified in both cadmium-and copper-resistant yeasts. However, the mechanism for the cadmiumspecific inducibility of the gene may be restricted to the cadmium-resistant strain.