The Fractionation of Human Plasma-Proteins: III. Purification of Complement Factors D and I Using Affinity Chromatography

✍ Scribed by R.G. Discipio

• Book ID: 115644820
• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 699 KB
• Volume: 5
• Category: Article
• ISSN: 1046-5928
• DOI: 10.1006/prep.1994.1028

✦ Synopsis

A purification protocol for complement factors (D) and I was designed as part of a larger scheme for the purification of more than 20 human plasma proteins. The key affinity step in the purification of both serine proteases, factors (D) and (I), was affinity chromatography using benzamidine Sepharose. The recoveries were 19 and (12 %) for factors (D) and (I), respectively. (c) 1994 Academic Press, Inc.