The fourth intracellular domain of G-protein coupling receptors: helicity, basicity and similarity to opsins

Delay of Intracellular Calcium Transient

Delay of Intracellular Calcium Transients Using a Calcium Chelator: Application to High-Throughput Screening of the Capsaicin Receptor Ion Channel and G-Protein-Coupled Receptors

✍ Stephan K. Grant; Alka Bansal; Anjalee Mitra; Scott D. Feighner; Ge Dai; Gregory 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 169 KB

Whole-cell functional assays are often used for high-throughput screening (HTS) of molecular targets such as ion channels and G-protein-coupled receptors. A common method for assaying the activity of these membrane proteins is to measure the change in intracellular calcium concentration upon recepto