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The Fluorescent Reaction between Berberine and DNA and the Fluorometry of DNA

โœ Scribed by Wen-You Li; Zu-Hong Lu


Book ID
102614372
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
112 KB
Volume
60
Category
Article
ISSN
0026-265X

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โœฆ Synopsis


Determination of DNA with berberine(BER) as a fluorescent probe was developed, based on the large fluorescence enhancement of BER in the presence of DNA and the poor fluorescence yield of BER in the absence of DNA. The calibration graph was linear in the range 0 -20 g/ml CT DNA. The limit of detection was 12 ng/ml CT DNA.


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