The Fluorescent Reaction between Berberine and DNA and the Fluorometry of DNA
โ Scribed by Wen-You Li; Zu-Hong Lu
- Book ID
- 102614372
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 112 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0026-265X
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โฆ Synopsis
Determination of DNA with berberine(BER) as a fluorescent probe was developed, based on the large fluorescence enhancement of BER in the presence of DNA and the poor fluorescence yield of BER in the absence of DNA. The calibration graph was linear in the range 0 -20 g/ml CT DNA. The limit of detection was 12 ng/ml CT DNA.
๐ SIMILAR VOLUMES
The interaction of the fluorescent dye thiazole orange (TO) with nucleic acids is characterized. It is found that TO binds with highest affinity to double-stranded (ds) DNA [log(K) ร 5.5 at 100 mM salt], about 5-10 times weaker to single-stranded polypurines, and further 10-1000 times weaker to sing