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The flow linear dichroism of DNA: Comparison with the bead-spring theory

✍ Scribed by Robert W. Wilson; John A. Schellman

Book ID
102761678
Publisher
Wiley (John Wiley & Sons)
Year
1978
Tongue
English
Weight
670 KB
Volume
17
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

The flow linear dichroism if T7 bacteriophage DNA has been obtained as a function of velocity gradient in neutral aqueous solutions containing 0.15__M__ Na^+^ or K^+^. The data are compared with the hydrodynamic bead‐spring theory of Rouse and Zimm using currently available spectroscopy, structural, and hydrodynamic parameters. Good agreement between theoretical and experimental isocline angle behavior is found. The magnitude of the measured linear dichroism is not in exact agreement with theory when presently accepted stiffness and optical parameters are used. Agreement is improved either by assuming a shorter persistence length or a less negative value for the limiting dichroism of DNA.

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