The fixation of avian chromosomes
β Scribed by Hance, Robert T.
- Publisher
- John Wiley and Sons
- Year
- 1925
- Tongue
- English
- Weight
- 305 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0003-276X
No coin nor oath required. For personal study only.
β¦ Synopsis
When a successful preservation f o r cytological study of mammalian tissue had been accomplished (Hance, '17), the hope arose that the other warm-blooded group-the birdsmight yield to the same treatment. The receipt of some beautifully fixed preparations of chick tissue cultures from Mrs. M. R. Lewis gave further expectation that equal results might be achieved with larger pieces of tissue put through the section method. Fortunately, the same methods that gave sharply defined chromosomes in mammalian cells gave comparable pictures with bird tissues (figs. 1 to 4).
The following technique was used first with chick embryos and later with the testes of adult roosters, pigeons, a robin, and sparrows.
F l e m i n g ' s strong solution plus about
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## Abstract The diploid number of chromosomes obtained from counts of anaphases of the first somatic mitosis is found to be fortyβfour. Of these, seven have terminal, thirtyβseven nonβterminal attachment, giving a distribution of seven rods, thirteen V's, and twentyβfour J's. The number is constant