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The fission yeast Schizosaccharomyces pombe in continuous culture

✍ Scribed by Dagmar Vraná

Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
310 KB
Volume
25
Category
Article
ISSN
0006-3592

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✦ Synopsis

The fission yeast Schizosaccharomyces pombe was cultivated in a chemostat at dilution rates of D = 0.03, 0.05, 0.10, and 0.20 h-l. After steady state had been reached, the amount of dry matter, number of cells, concentration of residual sugar, yield coefficient (Y), and some morphological properties of the cells were estimated. Curves reflecting the dry mass, number of cells, and cell mean volume show a changing coordination between the growth rate and the rate of cell division, with respect to D. In addition, it could be concluded that in dividing cells the cell septum is localized asymmetrically; Two nonidentical cells differing both in length and volume result. The degree of asymmetry is a function of the dilution rate.

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