The fimD gene required for cell surface localization of Escherichia coli type 1 fimbriae

✍ Scribed by Klemm, Per ;Christiansen, Gunna

• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 645 KB
• Volume: 220
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00260505

✦ Synopsis

The fimD gene of Escherichia coli K12 was shown to be necessary for surface localization of type 1 fimbriae, since deletion of the gene resulted in a virtually bald phenotype. The FimD protein was found to be located in the outer membrane. Expressed alone, this protein had a very deleterious effect on cell growth. The DNA sequence of the fimD gene was determined; the corresponding amino acid sequence of the FimD protein was compared with those of the PapC and FaeD proteins. A deletion derivative of FimD gave clues as to which parts of the protein were necessary for outer membrane integration.