𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The fate of mitochondrial DNAs ofmt+andmt−origin in gametes and zygotes ofChlamydomonas

✍ Scribed by M. -C. Beckers; C. Munaut; A. Minet; R. F. Matagne

Book ID
104722925
Publisher
Springer-Verlag
Year
1991
Tongue
English
Weight
747 KB
Volume
20
Category
Article
ISSN
0172-8083

No coin nor oath required. For personal study only.

✦ Synopsis

In order to study the mechanism responsible for the uniparental transmission of the mitochondrial genome in crosses between Chlamydomonas reinhardtii and C. smithii, we have analyzed the fate of mitochondrial DNA during gametogenesis, zygospore differentiation and sporulation by hybridization experiments. Both mt+ and mt- gametes contain the same amount of mitochondrial DNA and the two parental genomes persist for several days in the zygotes. The DNA of mt+ origin is slowly eliminated during the period of zygote maturation. Light is required for total elimination of mt+ mitochondrial DNA in the zygospores. Using appropriate restriction enzymes, we have been unable to detect methylation of the mitochondrial DNA during gametogenesis or zygospore formation. The possibility that the mt+ mitochondria themselves are specifically eliminated in the course of zygote maturation is discussed.

📜 SIMILAR VOLUMES

Mitochondrial DNA-like sequences in the
Mitochondrial DNA-like sequences in the nucleus (NUMTs): Insights into our African origins and the mechanism of foreign DNA integration
✍ Dan Mishmar; Eduardo Ruiz-Pesini; Martin Brandon; Douglas C. Wallace 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 186 KB

## Communicated by Nobuyoshi Shimizu Nuclear mitochondrial DNA sequences (NUMTs) are common in eukaryotes. However, the mechanism by which they integrate into the nuclear genome remains a riddle. We analyzed 247 NUMTs in the human nuclear DNA (nDNA), along with their flanking regions. This analysi

A direct study of the relative synthesis
A direct study of the relative synthesis of petite and grande mitochondrial DNA in zygotes from crosses involving suppressive petite mutants ofSaccharomyces cerevisiae
✍ Paul Chambers; Elliot Gingold 📂 Article 📅 1986 🏛 Springer-Verlag 🌐 English ⚖ 726 KB

Work in recent years has produced indirect evidence to support the view that the phenomenon of suppressiveness in yeast is the result of the ability of the petite mtDNA to out-replicate the wild-type genome. We have developed a method, based on fluorography of gels containing restriction fragments o