✦ LIBER ✦
The farnesyl group activates Ras toward guanine nucleotide exchange catalyzed by the SOS protein
✍ Scribed by Paul McGeady; Emilio Porfiri; Michael H. Gelb
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 254 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0960-894X
No coin nor oath required. For personal study only.
✦ Synopsis
The farnesyl group and a number of its analogs were enzymatically incorporated into H-Ras and K-Ras in vitro in order to study the prenyl group dependence of the hSOSl-catalyzed nucleotide exchange of Ras. Farnesylation of H-Ras and K-Ras in the absence of further processing was sufficient to facilitate the exchange although fully processed Ras showed a somewhat higher rate of exchange. H-Ras containing 15-carbon analogs of the farnesyl group were good substrates for hSOS 1, but geranylated-H-Ras was not.