The extracellular polysaccharide of Pichia (Hansenula) holstii NRRL Y-2448: The structure of the phosphomannan backbone
✍ Scribed by Lesley A.S. Parolis; Jens Ø. Duus; Haralambos Parolis; Morten Meldal; Klaus Bock
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 800 KB
- Volume
- 293
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
The phosphomannan core of the exopolysaccharide of Pichia (Hansenula) holstii NRRL Y-2448 was isolated after hydrolytic removal of the oligosaccharide phosphate side-chains. The core polysaccharide and its dephosphorylated derivative were subjected to extensive 1D and 2D NMR spectroscopy which yielded information on the linkage sites and on the sequence of the mannosyl residues in the major oligosaccharide repeating unit. The most probable structure for the repeating unit was
A semiquantitative conformational analysis was performed by Monte Carlo simulations and the result was confirmed by comparison with the experimentally determined NMR data. The distance distribution for the phosphate groups was determined from the modelling and was found to cover the expected range of distances for phosphorylated high-mannose oligosaccharides.