The expression of placental-type glutathione S-tranferase (GST-π) in human cutaneous squamous cell carcinoma and normal human skin

✍ Scribed by K. Shimizu; F. Toriyama; H. Yoshida

• Publisher: Springer
• Year: 1995
• Tongue: English
• Weight: 851 KB
• Volume: 425
• Category: Article
• ISSN: 1432-2307
• DOI: 10.1007/bf00199348

✦ Synopsis

The expression of human placental-type glutathione S-transferase (GST-~) was investigated in human cutaneous squamous cell carcinoma (SCC) and normal skin using Northern blot and immunohistochemical analysis. In Northern blot examination, the expression of GST-~ transcript was recognized in all instances, and SCC showed a significantly higher expression of GST-rc than normal skin. In immunohistochemical examination, GST-~ was stained well in the cytoplasm of all cells of the stratum granulosum, many cells of the stratum spinosum and a few cells of the stratum basale in normal skin. Some cells of the stratum spinosum and almost all cells of the stratum basale showed only a weakly positive or almost negative reaction for GST-r~. No nuclear staining of GST-r~ was obvious in normal epidermal cells. In SCC, many cells showed strong positivity for GST-r~ in the cytoplasm, and some were obviously accompanied by nuclear staining of GST-r~. These findings suggest that GST-~ exists mainly in many cells in the upper layers of the normal epidermis and that GST-rc is involved in the process of carcinogenesis.