The expression of Na+/K+-ATPase β-subunit cRNA injected intoXenopusoocytes is affected by coinjection with α-subunit cRNA
✍ Scribed by S. Ueno; K. Takeda; S. Noguchi; F. Izumi; M. Kawamura
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 856 KB
- Volume
- 148
- Category
- Article
- ISSN
- 0022-2631
No coin nor oath required. For personal study only.
✦ Synopsis
The cRNA for Torpedo californica Na+/K(+)-ATPase beta-subunit (cRNA beta) was injected into Xenopus oocytes alone or with the cRNA for the Na+/K(+)-ATPase alpha-subunit (cRNA alpha). When cRNA beta was injected alone, the amount of the beta-subunit that accumulated in oocytes increased with increasing amounts of injected cRNA beta. When cRNA beta and cRNA alpha were injected simultaneously, less beta-subunit accumulated than when cRNA beta was injected alone, whereas the Na+/K(+)-ATPase activity increased markedly. The decrease in the accumulation of the beta-subunit was dose-dependent upon the cRNA alpha. The mutant beta-subunit unable to assemble with the alpha-subunit accumulated in oocytes independently of cRNA alpha, suggesting that post-translational control mechanisms may serve to reduce the accumulation of the beta-subunit.