The expression of integrin subunits ?6 and ?4 by corneal epithelial cells on modified hydrogel surfaces
✍ Scribed by Wu, X. Y. ;Trinkaus-Randall, V.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 295 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
Our goal was to quantitate the expression and and localization of integrin subunits was surface dependent. localization of integrin subunits ␣6 and 4 by corneal epithe-Alpha6 and 4 proteins were localized along the basal surface lial cells on defined synthetic substrates. Previously we dem-of nonpermeabilized cells cultured on laminin, on surfaces onstrated that the cytoplasmic pH and translocation of the with amine moieties, and on those with amine and carboxyl ␣6 integrin subunit to the cell membrane was modified by moieties. The level of diffuse cytoplasmic staining increased ionic interactions. These results suggest that changes in the with the presence of carboxyl moieties. Alpha6 and 4 inteionic interactions at the cell-substrate interface not only alter grin subunits were negligible when the surfaces contained the intracellular milieu but ultimately affect the expression carboxyl moieties alone. The expression of ␣6 and 4 mRNA of adhesion proteins. To test this hypothesis, hydroxyethyl-was higher on surfaces containing amine moieties than on methacrylate (hema) hydrogels were modified by the addi-surfaces containing only carboxyl moieties. These results intion of amines (N,N-dimethylaminoethylmethacrylate) or dicate that the characteristics of the substrate and the recarboxyl moieties (methacrylic acid). Changes in the distribu-sulting cell-matrix interaction alter protein and mRNA tion of mRNA and protein were monitored using confocal expression of integrin subunits. © 1997 John Wiley & Sons, laser scanning microscopy. The steady state level of integrin Inc. J Biomed Mater Res, 37, 166-175, 1997. mRNA was evaluated, and the results indicate that while the plating efficiency was identical on all surfaces, the expression