The expression of I-Edmolecules in F1hybrid mice detected with antigen-specific, I-Ed-restricted cloned T-cell lines
✍ Scribed by Patricia J. Conrad; Charles A. Janeway
- Publisher
- Springer-Verlag
- Year
- 1984
- Tongue
- English
- Weight
- 548 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0093-7711
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✦ Synopsis
The expression of polymorphic determinants on I-E molecules is largely dependent on allelic variation in the E~ chain. We have previously analyzed the expression of E~ and E~ chains in F 1 hybrid mice by a combination of techniques, and have shown that functional variation detected by the responsiveness of cloned T-cell lines specific for these molecules correlates well with serological determination of E B expression. In the present study, we have extended our analysis to E~ expression in F~ hybrid mice. We show that E~ is relatively poorly expressed in three F~ combinations: H-2 d x H-2 b, H-2 d x H-2 s, and H-2 d x H-2". The former two crosses express E~ chains from the H-2 d parent only; When recombinant strains carrying E~ or E} and an active E~ gene are used, E~ expression is significantly increased. On the other hand, H-2 u mice synthesize E~ chains; the poor expression of E~ chains in this F~ hybrid apparently reflects the strong preferential association of E} chains with all E~ molecules thus far analyzed. These results confirm that E~ chains compete for binding to E~ chains and that preferential association of different alMic forms of E~ chains with E~ • chains is a generalized phenomenon. They also illustrate the importance of the rate of biosynthesis of Ia chains for cell-surface expression.