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THE EXPRESSION OF ESTROGEN RECEPTOR AND ESTROGEN EFFECT IN MBA-15 MARROW STROMAL OSTEOBLASTS

✍ Scribed by A. SHAMAY; V. KNOPOV; D. BENAYAHU

Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
415 KB
Volume
20
Category
Article
ISSN
1065-6995

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✦ Synopsis

MBA-15, a marrow stromal-derived cell line, was shown to express an estrogen receptor. This finding was confirmed by in situ hybridization and receptor binding assay. An exposure to estrogen (10 12 -10 6 ) in a dose response manner resulted in a decrease of cell proliferation as measured by MTT assay. Cell function was measured by enzymatic activities of two osteoblastic markers, CD10/NEP and alkaline phosphatase. These enzymatic activities were elevated following the estrogen treatment. This model enabled direct evaluation of the estrogen effect on stromal osteoblast cells.

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