The expression of cecropin peptide in transgenic tobacco does not confer resistance toPseudomonas syringaepvtabaci
✍ Scribed by Robin Hightower; Catherine Baden; Eva Penzes; Pamela Dunsmuir
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 758 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0721-7714
No coin nor oath required. For personal study only.
✦ Synopsis
We used in vitro growth inhibition assays to demonstrate that synthetic cecropin protein has potent activity against a range of plant pathogenic bacteria. We then prepared transgenic tobacco plants which express cecropin mRNA and protein. We have used Pseudomonas syringae pv tabaci infection of these transgenic tobacco as a model system to evaluate whether the plants which express cecropin protein also have increased tolerance to infection. We found no dramatic difference in disease response between plants which are expressing cecropin protein and control plants which were derived from the transformation with a binary vector which did not carry the gene encoding cecropin protein.