α-lactalbumin mutant acting as lysozyme
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Yuming Xue; Jian-Ning Liu; Ziyong Sun; Zhong Ma; Chunlei Wu; Dexu Zhu
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Article
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2000
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John Wiley and Sons
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English
⚖ 188 KB
A mutant of ␣-lactalbumin was expressed and purified, in which His32, Thr33, Glu49, Ile59, Val99, and Tyr103 were substituted by Leu32, Glu33, Asp49, Trp59, Asn99, and Ala103, respectively, to create a catalytic site of lysozyme in ␣-lactalbumin. The mutant catalyzed hydrolysis of the synthetic subs