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The estimation of turnover of spermidine in Anacystis nidulans

✍ Scribed by Linda A. Guarino; Seymour S. Cohen

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
328 KB
Volume
95
Category
Article
ISSN
0003-2697

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✦ Synopsis

The turnover of spermidine in Anacystis nidulans was studied using [2-Ylmethionine to prelabel intracellular spermidine. It was found that there is essentially neither excretion nor degradation of spermidine in exponentially gr0wingAnacysti.s nidukms.

Spermidine was degraded rapidly in stationary phase cells. The half-life of specific activity of spermidine in exponential phase was 8.3 h, a period similar to that of the doubling time (7.5 h) of the bacterium. The rate of synthesis of spermidine was calculated to be 0.04 nmol/lOn cells/h.

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