The estimation of sphingolipids by gas chromatography-chemical ionization mass spectrometry of their derived aldehydes with particular reference to the ceramides of children's plasma
β Scribed by A. Poulos; C. Hann; G. Phillipou; A.C. Pollard
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 379 KB
- Volume
- 97
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
A method for the estimation of sphingolipids based on the quantitation, by gas-liquid chromatography/chemical ionization, of the long-chain aldehydes released from the native lipids, is described. The method is highly sensitive (6-7 pmol per injection), specific, and can, in addition, provide information on the chain length and degree of unsaturation of the long-chain bases.
π SIMILAR VOLUMES
This study describes a rapid and simple method to determine short-chain fatty acid (SCFA) concentrations and their isotopic enrichments (M(0) + 1 and M(0) + 2) in human plasma. Sample preparation involves SCFA extraction and derivatization with 1-(tert-butyldimethylsilyl)imidazole. Gas chromatograph