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The estimation of sphingolipids by gas chromatography-chemical ionization mass spectrometry of their derived aldehydes with particular reference to the ceramides of children's plasma

✍ Scribed by A. Poulos; C. Hann; G. Phillipou; A.C. Pollard


Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
379 KB
Volume
97
Category
Article
ISSN
0003-2697

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✦ Synopsis


A method for the estimation of sphingolipids based on the quantitation, by gas-liquid chromatography/chemical ionization, of the long-chain aldehydes released from the native lipids, is described. The method is highly sensitive (6-7 pmol per injection), specific, and can, in addition, provide information on the chain length and degree of unsaturation of the long-chain bases.


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