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The establishment and application of a semi-nested PCR assay for the detection of Mycoplasma ovis

✍ Scribed by Song, Weijiao; Song, Qiqi; He, Lan; Zhou, Yanqin; Zhao, Junlong

Book ID: 122180756
Publisher: Elsevier Science
Year: 2014
Tongue: English
Weight: 673 KB
Volume: 119
Category: Article
ISSN: 0921-4488
DOI: 10.1016/j.smallrumres.2014.03.001

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## Abstract Direct culture is the most common way to reliably detect mycoplasma, but it is not practical for the qualitative control of cell therapeutics because of the elaborate culture medium, the prolonged incubation time, and the large sample volumes. Here, we chose two alternative methods usin