The essentiality of His-47 and the N-terminal region for the binding of 8-anilinonaphthalene-1-sulfonate with Taiwan cobra phospholipase A2

The structural and functional contributi

The structural and functional contribution of N-terminal region and His-47 on Taiwan cobra phospholipase A2

✍ Pei-Hsiu Kao; Ku-Chung Chen; Shinne-Ren Lin; Long-Sen Chang 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 384 KB

Modification of His-47 and removal of the N-terminal octapeptide caused a different effect on the structure of Naja naja atra (Taiwan cobra) phospholipase A2 (PLA2). Unlike native enzyme, Ca2+ induced an alteration in the structural flexibility of His-modified PLA2. Moreover, the spatial positions o