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The Escherichia coli O149 rfb gene cluster and its use for the detection of porcine E. coli O149 by real-time PCR

โœ Scribed by Priti Goswami; Carlton Gyles; Robert Friendship; Cornelis Poppe; Evguenii Vinogradov; Patrick Boerlin

Book ID
113944063
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
221 KB
Volume
141
Category
Article
ISSN
0378-1135

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Molecular beacons (MBs) are oligonucleotide probes that fluoresce upon hybridization. In this paper, we described the development of a real-time PCR assay to detect the presence of Escherichia coli O157:H7 using these fluorogenic reporter molecules. MBs were designed to recognize a 26-bp region of t