The epstein-barr-virus-encoded membrane protein LMP but not the nuclear antigen EBNA-1 induces rejection of transfected murine mammary carcinoma cells

✍ Scribed by Pankaj Trivedi; Maria G. Masucci; Gösta Winberg; George Klein

• Book ID: 102868826
• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: French
• Weight: 761 KB
• Volume: 48
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910480527

✦ Synopsis

The EBV-encoded membrane protein (LMP) and the nuclear antigen EBNA-I were compared for their capacity to induce rejection of transfected murine mammary carcinoma cells in syngeneic hosts. The tumorigenic potential of stable

LMP and E B N A -I expressing sublines of t h e A C A (H-2'

)-derived mammary carcinoma line S6C was tested in pre-immunized syngeneic and semi-syngeneic animals. LMP expressing S6C cells elicited a strong rejection response as demonstrated by the lower tumor take and slower growth in immunized vs. control mice. In contrast, EBNA-I-expressing cells were non-immunogenic in syngeneic hosts and in one semi-syngeneic F,-hybrid. Rejection in 2 additional F,-hybrids did not appear to be due to EBNA-I-specific immune responses. Our findings support the hypothesis that the escape of EBV carrying tumor cells from EBV-specific immune surveillance may be facilitated by the fact that viral gene expression is limited to EBNA-I.