The Epithelial-to Mesenchymal Transition: Methods and Protocols

✍ Scribed by Kyra Campbell, Eric Theveneau

• Publisher: Springer US;Humana
• Year: 2021
• Tongue: English
• Leaves: 425
• Series: Methods in Molecular Biology 2179
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This volume details a comprehensive range of methods for imaging epithelial-to-mesechymal transition (EMT)/MET in in vivo systems, and methods to leverage these systems to dissect the underlying mechanisms. Chapters guide readers through studying different features of epithelial-mesenchymal plasticity, past and future research of the EMT, in vivo systems, and in vivo imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, The Epithelial-to Mesenchymal Transition: Methods and Protocols aims to provide methods in EMT will help to unite and drive research in this exciting field forwards.

✦ Table of Contents

Front Matter ....Pages i-xv
Front Matter ....Pages 1-1
A Spectrum of Cell States During the Epithelial-to-Mesenchymal Transition (Erica J. Hutchins, Marianne E. Bronner)....Pages 3-6
Perspective on Epithelial-Mesenchymal Transitions in Embryos (David R. McClay)....Pages 7-12
EMT, One of Many Morphological Transitions in Cellular Phase Space (Denise J. Montell)....Pages 13-18
Are You Interested or Afraid of Working on EMT? (M. Angela Nieto)....Pages 19-28
Partial EMT/MET: An Army of One (Sofiane Hamidi, Hiroki Nagai, Guojun Sheng)....Pages 29-33
EMT: An Update (Jean Paul Thiery)....Pages 35-39
Front Matter ....Pages 41-41
Mesenchymal-to-Epithelial Transitions in Development and Cancer (John-Poul Ng-Blichfeldt, Katja Röper)....Pages 43-62
Front Matter ....Pages 63-63
Live Imaging of Epithelial-Mesenchymal Transition in Mesoderm Cells of Gastrulating Drosophila Embryos (Lingkun Gu, Mo Weng)....Pages 65-77
Zebrafish Neural Crest: Lessons and Tools to Study In Vivo Cell Migration (Zain Alhashem, Macarena Alvarez-Garcillan Portillo, Mint Ravinand Htun, Anton Gauert, Luis Briones Montecinos, Steffen Härtel et al.)....Pages 79-106
Live Imaging of the Neural Crest Cell Epithelial-to-Mesenchymal Transition in the Chick Embryo (Mary Cathleen McKinney, Paul M. Kulesa)....Pages 107-114
Exploiting Drosophila melanogaster Wing Imaginal Disc Eversion to Screen for New EMT Effectors (Sofia Golenkina, Rosemary Manhire-Heath, Michael J. Murray)....Pages 115-134
Visualizing Mouse Embryo Gastrulation Epithelial-Mesenchymal Transition Through Single Cell Labeling Followed by Ex Vivo Whole Embryo Live Imaging (Wallis Nahaboo, Bechara Saykali, Navrita Mathiah, Isabelle Migeotte)....Pages 135-144
Fluorescence Recovery After Photobleaching to Study the Dynamics of Membrane-Bound Proteins In Vivo Using the Drosophila Embryo (Joshua Greig, Natalia A. Bulgakova)....Pages 145-159
Methods to Generate and Assay for Distinct Stages of Cancer Metastasis in Adult Drosophila melanogaster (Jamie Adams, Andreu Casali, Kyra Campbell)....Pages 161-170
Front Matter ....Pages 171-171
4D Live Imaging and Analysis of Chick Embryo Somites (Gi Fay Mok, James McColl, Andrea Münsterberg)....Pages 173-181
In Vivo Analysis of the Mesenchymal-to-Epithelial Transition During Chick Secondary Neurulation (Elena Gonzalez-Gobartt, Guillaume Allio, Bertrand Bénazéraf, Elisa Martí)....Pages 183-197
Multiscale In Vivo Imaging of Collective Cell Migration in Drosophila Embryos (Gordana Scepanovic, Alexandru Florea, Rodrigo Fernandez-Gonzalez)....Pages 199-224
Front Matter ....Pages 225-225
Methods to Generate Tube Micropatterns for Epithelial Morphogenetic Analyses and Tissue Engineering (Minerva Bosch-Fortea, Fernando Martín-Belmonte)....Pages 227-242
CAFs and Cancer Cells Co-Migration in 3D Spheroid Invasion Assay (Sefora Conti, Takuya Kato, Danielle Park, Erik Sahai, Xavier Trepat, Anna Labernadie)....Pages 243-256
Using Xenopus Neural Crest Explants to Study Epithelial-Mesenchymal Transition (Nadège Gouignard, Christian Rouvière, Eric Theveneau)....Pages 257-274
Xenopus Deep Cell Aggregates: A 3D Tissue Model for Mesenchymal-to-Epithelial Transition (Hye Young Kim, Lance A. Davidson)....Pages 275-287
Molecular Tension Microscopy of E-Cadherin During Epithelial-Mesenchymal Transition (Helena Canever, Pietro Salvatore Carollo, Romain Fleurisson, Philippe P. Girard, Nicolas Borghi)....Pages 289-299
Front Matter ....Pages 301-301
Methodologies for Following EMT In Vivo at Single Cell Resolution (Abdull J. Massri, Geoffrey R. Schiebinger, Alejandro Berrio, Lingyu Wang, Gregory A. Wray, David R. McClay)....Pages 303-314
Isolation and Identification of EMT Subtypes (Robert J. Norgard, Ben Z. Stanger)....Pages 315-326
Studying the Metabolism of Epithelial-Mesenchymal Plasticity Using the Seahorse XFe96 Extracellular Flux Analyzer (Sugandha Bhatia, Erik W. Thompson, Jennifer H. Gunter)....Pages 327-340
Inducing Sequential Cycles of Epithelial-Mesenchymal and Mesenchymal-Epithelial Transitions in Mammary Epithelial Cells (Cecile Davaine, Eva Hadadi, William Taylor, Annelise Bennaceur-Griscelli, Hervé Acloque)....Pages 341-351
Analysis of Cellular EMT States Using Molecular Biology and High Resolution FISH Labeling (Noémie Kempf, Fatima Moutahir, Isabelle Goiffon, Sylvain Cantaloube, Kerstin Bystricky, Anne-Claire Lavigne)....Pages 353-383
Mathematical Modeling of Plasticity and Heterogeneity in EMT (Shubham Tripathi, Jianhua Xing, Herbert Levine, Mohit Kumar Jolly)....Pages 385-413
Control of Cell Migration Using Optogenetics (Leo Valon, Simon de Beco)....Pages 415-425
Back Matter ....Pages 427-429

✦ Subjects

Life Sciences; Cell Biology