AMPLIFICATION OF SPECIFIC mRNA FROM A SI
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BICKNELL, GARETH R.; SHAW, JACQUI A.; PRINGLE, J. HOWARD; FURNESS, PETER N.
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Article
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1996
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John Wiley and Sons
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English
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A method has been developed by which single human glomeruli may be plucked from fresh renal biopsies under direct vision, followed by separation of mRNA using oligo-dT-linked paramagnetic beads. The mRNA was amplified by reverse transcription and polymerase chain reaction (RT-PCR). Primers for a var