The enzymic determination of d-mannitol with mannitol dehydrogenase from Agaricus bisporus

✍ Scribed by Stephen Berezenko; Robert J. Sturgeon

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 354 KB
• Volume: 216
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(92)84185-u

✦ Synopsis

Mannitol dehydrogenase (D-mannitol:NADP+ 2-oxidoreductase, EC 1. I. 1.138), isolated from the sporocarps of Agaricus biqmrus, has been purified 120-fold following fractionation with protamine sulphate, followed by hydrophobic and affinity chromatography. D-Mannitol and u-fructose appear to be the only substrate and products involved in the reaction, having Km values of 7.5 and 9.8mM, respectively. The purified enzyme has been used for the determination of D-mannitol and also the D-mannose content of glycoproteins and polysaccharides following the liberation of that hexose and reduction to D-mannitol.