✦ LIBER ✦
The enhanced production of hyaluronic acid by cultured rat fibroblast cells treated with cyclic AMP and its dibutyryl derivative
✍ Scribed by Hideki Koyama; Mikio Tomida; Tetsuo Ono
- Publisher
- John Wiley and Sons
- Year
- 1976
- Tongue
- English
- Weight
- 730 KB
- Volume
- 87
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Abstract
Cells of a newly established rat fibroblast line (SEN) in culture synthesize mucopolysaccharides, which have been identified as hyaluronic acid, chondroitin‐4‐sulfate and heparan sulfate. Treatment of the cells with adenosine 3′:5′‐cyclic monophosphate resulted in a marked stimulation of production of hyaluronic acid, but not of the other mucopolysaccharides. Treated cells also showed increased activity of hyaluronic acid synthetase, a reduction in growth rate, and morphological alteration. In addition, 5‐bromodeoxyuridine was found to counteract greatly the cyclic AMP effect.