The endonucleolytic reaction catalysed by the EcoRI restriction enzyme is inhibited when the enzyme is in excess over substrate

Kinetics of substrate reaction during ir

Kinetics of substrate reaction during irreversible modification of enzyme activity where the modifier is not in great excess of the enzyme

✍ Kang-Yuan Zhao; Chen-Lu Tsou 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 586 KB

The kinetic approach previously developed (Tsou 1988, Adv. Enzymok 61,381-386) for the substrate reaction in the presence of modifier is based on the supposition that [Yo]>> [Eo]. However, the presence of the modifier in large excess of the enzyme sometimes leads to a rate of modification which is t