The elucidation of the effect of ammonium chloride on pyruvate distribution and pyruvate dehydrogenase interconversion in isolated rat hepatocytes
✍ Scribed by Elżbieta I. Wałajtys-Rode; Katarzyna A. Nałȩcz; Anna Sterniczuk; Anna B. Wojtczak
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 727 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0020-711X
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📜 SIMILAR VOLUMES
## Abstract Thymocytes incubated as cell suspensions in vitro are known to be markedly dependent upon added glucose for maintenance of maximal rates of incorporation of radiolabelled amino acids into protein. This requirement is only partially satisfied by other added substrates, such as pyruvate.
Periportal (pp) and perivenous (pv) hepatocyte populations were separated using a two-directional closed perfusion technique with selective addition of collagenase either to direct or retrograde perfusions (Väänänen, H. et al., Liver 1983; 3:131). The activity of GPT in hepatocytes from the pp-area