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The electrochemical aspects of some biochemical systems—XI. The coulokinetic behaviour of metabolizing organisms in the presence of cyanide

✍ Scribed by M.J. Allen

Publisher
Elsevier Science
Year
1967
Tongue
English
Weight
411 KB
Volume
12
Category
Article
ISSN
0013-4686

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✦ Synopsis

The effects of cyanide as an inhibitor of the cytochrome system and formic dehydrogenlyase has been correlated with the coulokinetic data obtained from the metabolism of various substrates by E. coli in the presence of this inhibitor.

A negligible coulombic output was obtained with formic acid in the presence of cyanide. This finding reaffirmed the direct relationship between metabolic activity and coulombic output. The potentiated output obtained with glucose as compared to the attenuated output manifested by fructose in the presence of cyanide indicates again the possibility that glucose and fructose are not metabolized via the same metabolic pathway. As cyanide inhibits pyruvate oxidation the lower coulombic output obtained with this substrate is not unexpected. In the presence of cyanide, gluconolactone apparently was not metabolized by E. coli in a normal manner. This implicates this poison as a blocking agent for the pentose phosphate pathway.

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The electrochemical aspects of some bioc
The electrochemical aspects of some biochemical systems—V. The electrochemical differentiation between aerobic and anaerobic metabolic paths
✍ M.J. Allen 📂 Article 📅 1964 🏛 Elsevier Science 🌐 English ⚖ 309 KB

An electrochemical method is described by which it appears possible to distinguish aerobic from anaerobic pathways in the oxidation of various substrates by E. coli. The results obtained from the oxidation of glucose indicate that 23 per cent of the oxidation occurs via the "pcntose phosphate cycle"