The effects ofN-ethylmaleimide on extracellularly and intracellularly generated chemiluminescence in neutrophils indicate that the rate of deactivation of NADPH-oxidase is higher when the oxidase system is localized on the plasma membrane than when it is localized on the phagosomal membrane
✍ Scribed by Dahlgren, Claes ;Sundqvist, Tommy
- Publisher
- John Wiley and Sons
- Year
- 1991
- Weight
- 510 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0884-3996
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✦ Synopsis
Sustained generation of reactive oxygen metabolites following respiratory burst activation in neutrophils is a result of continued replenishment o f a pool of active NADPH-oxidase. The sulphydryl-modifying reagent N-ethylmaleimide (NEM) has been shown t o be without effect on the turnover of activated NADPH-oxidase but t o inhibit the replenishment o f active oxidase molecules (Akard eta/., 1988). N E M was thus used t o determine the rate of deactivation o f extracellularly and intracellularly generated chemiluminescence in human neutrophils. We have shown that deactivation is more rapid when activation leads to a release o f oxygen metabolites (extracellular chemiluminescence) than when the metabolites are generated intracellularly. The results indicate that the rate of deactivation o f NADPH-oxidase is higher when the oxidase system is localized o n the plasma membrane than when it is localized o n the phago-soma1 membrane.