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The effects of tumour extract on macrophage proliferation in lymph-nodes

โœ Scribed by I. Carr; P. Price; S. Westby


Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
207 KB
Volume
120
Category
Article
ISSN
0022-3417

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โœฆ Synopsis


Injection of a soluble tumour extract induces mitosis in sinus macrophages of the draining lymph-node. This is not abolished by irradiation; the stimulus also induces monocytosis somewhat later. These findings make it likely that the stimulus induces mitosis in the resident lymph-node macrophage population.


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A modified version of the local lymph node assay (LLNA) is presented, using bromodeoxyuridine to label proliferating lymphocytes. Cell counting is done on mid-sagittal sections of individual nodes under light microscopy. Two irritants (sodium lauryl sulfate and salicylic acid), four allergens of var