The effects of silver nitrate, colchicine, cupric sulfate and genotype on the production of embryoids from anthers of tetraploid wheat (Triticum turgidum)
✍ Scribed by Maryam Ghaemi; Ahmad Sarrafi; Gilber Alibert
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 377 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0167-6857
No coin nor oath required. For personal study only.
✦ Synopsis
Anther culture of four tetraploid wheat (Triticum turgidum) genotypes was studied using ten different culture medium treatments in a randomized block design with four replicates. Each replicate consisted of 2 pots with 3 plants. Anther donor plants were grown in a greenhouse with a 16 h day / 8 h night at 25°C and 15°C, respectively. The first treatment which was considered as the control, was potato 2 medium modified by adding 0.5 g 1-1 glutamine and solidified by gelrite (4 g 1-1). The nine test treatments differed from the control by addition of 3 different concentrations of silver nitrate (1, 2.5 and 5 mg l-l), colchicine (10, 100 and 200 mg 1 -l) or cupric sulfate (2, 5 and 10 mg 1-1). The study of about 2000 anthers per genotype and treatment showed that both genotype and treatment affected embryoid formation. The presence of cupric sulfate (10 mg 1-1) and silver nitrate (2.5 and 5 mg 1-1) usually increased the frequency of embryoid formation in 3 genotypes out of the 4 studied. On the contrary, colchicine had a significant and negative effect on anther culture responses for three out of the four genotypes studied. Because of the large genotype x medium interaction, it is very difficult to identify the best medium for embryo production by all genotypes studied.