✦ LIBER ✦

The effects of preparative procedures for scanning electron microscopy on the size of isolated lymphocytes

✍ Scribed by Schneider, Gary B.

Publisher: Wiley (John Wiley & Sons)
Year: 1976
Tongue: English
Weight: 612 KB
Volume: 146
Category: Article
ISSN: 0002-9106
DOI: 10.1002/aja.1001460105

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✦ Synopsis

Abstract

The diameter of lymphocytes, isolated from a sample of peripheral blood by Ficoll‐Hypaque sedimentation, was determined after eight different steps in the preparative procedure for scanning electron microscopy (SEM). There was a 43% total reduction in mean cellular diameter of the lymphocytes studied, the diameter dropping from 8.4 μm prior to fixation, to a final diameter of 4.8 μm in the cells examined under the scanning electron microscope. The bulk of this shrinkage occurred during critical‐point drying. In view of this finding, caution should be exercised in describing detailed cell surface morphology by SEM when similar preparative procedures are employed.

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