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The Effects of pKa Tuning on the Thermodynamics and Kinetics of Folding: Design of a Solvent-Shielded Carboxylate Pair at the a-Position of a Coiled-Coil

✍ Scribed by Lau, Wai Leung; DeGrado, William F.; Roder, Heinrich


Book ID
119207482
Publisher
Biophysical Society
Year
2010
Tongue
English
Weight
437 KB
Volume
99
Category
Article
ISSN
0006-3495

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The kinetics of folding of the two forms of unfolded ribonuclease A have been measured as a function of solvent viscosity by adding either glycerol or sucrose. The aim is to find out if either reaction is rate limited by segmental motion whose rate depends on external friction. The fast folding rea