The effects of p53 status and human papillomavirus infection on the clinical outcome of patients with Stage IIIB cervical carcinoma treated with radiation therapy alone

✍ Scribed by Hitoshi Ishikawa; Norio Mitsuhashi; Hideyuki Sakurai; Katsuya Maebayashi; Hideo Niibe

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 144 KB
• Volume: 91
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(20010101)91:1<80::aid-cncr11>3.0.co;2-e

✦ Synopsis

BACKGROUND.

It has been suggested that the p53 tumor suppressor gene regulates the radiosensitivity in human malignancies after irradiation; however, in cervical carcinoma, the role of the p53 gene is still unclear because of inactivation of functional p53 by infection with human papillomavirus (HPV). The objective of this study was to clarify the effects of p53 status and HPV infection on the clinical outcome of patients with cervical carcinoma after undergoing radiation therapy.

METHODS. Fifty-two patients with International Federation of Gynecology and

Obstetrics Stage IIIB squamous cell carcinoma of the cervix who received radiation therapy alone were reviewed. The combination of external beam irradiation therapy and three sessions of intracavity brachytherapy irradiation was performed for all patients. Genomic DNA extracted from paraffin embedded tissues was examined for HPV types 16, 18 and 33 by the polymerase chain reaction (PCR) method and for p53 status by PCR-single-strand conformation polymorphism (PCR-SSCP) technique. The effects of HPV infection, p53 status, and other parameters on clinical outcome were investigated by univariate analysis. LN: lymph node; IB: intracavity brachytherapy