The effects of mass accuracy, data acquisition speed, and search algorithm choice on peptide identification rates in phosphoproteomics

Effect of Matrix-Assisted Laser Desorpti

Effect of Matrix-Assisted Laser Desorption Ionization-Time of Flight-Mass Spectrometric Analysis Conditions and Accuracy Rating on Protein Database Search in Peptides Mass Fingerprint Identify Proteins

✍ Yong-Bo PENG; Yong-Ping MA; Shi-Jia DING; Yong-Peng XIA; Zong-Yin QIU 📂 Article 📅 2009 🏛 Elsevier Science 🌐 Chinese ⚖ 308 KB

To inspect the effect of main MS analysis conditions and data accuracy through matrix-assisted laser desorption ionization-time of flight-mass spectrometry and to obtain peptides mass fingerprint (PMF) for protein search results. BSA had been separated by SDS-PAGE, hydrolyzed by trypsin, and identif