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The effects of KNO3concentration in callus induction medium for wheat anther culture

✍ Scribed by Guo Hong Feng; Junwen Ouyang

Publisher
Springer
Year
1988
Tongue
English
Weight
440 KB
Volume
12
Category
Article
ISSN
0167-6857

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✦ Synopsis

KNO3 concentration was found to significantly affect the anther culture of wheat (Triticum aestivum L.). When KNO3 was increased from 0 to 15mM (in cultivar Jinghua 1) or from 10 to 15 mM (in cultivars 2531-10, Xiaoyan 759 and Norin 10), the callus induction frequency increased significantly. When KNO3 was increased further above 20 raM, the callus induction frequency decreased significantly in all the tested cultivars. The subsequent frequency of green plantlet regeneration increased significantly, and the ratio of green to albino regenerants increased sharply when KNO3 concentration increased. Further experiments found that the decrease of callus induction frequency in the medium with too much KNO 3 might be caused by NOr ion alone, while the effect of KNO3 on green plantlet regeneration might be caused by both K + and NOf ions, and that the effects of NOr concentration were independent of NH; concentration in the medium.

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