The effects of kinetin and contaminating bacteria on the incorporation of32P-orthophosphate into various fractions of nucleic acid extracted from radish leaves

✍ Scribed by A. N. Burdett; P. F. Wareing

• Publisher: Springer-Verlag
• Year: 1968
• Tongue: English
• Weight: 505 KB
• Volume: 81
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00385518

✦ Synopsis

By comparing 3~P-orthophosphate incorporation into nucleic acid extracts of sterile and non-sterile radish leaf discs, it was shown that contaminating bacteria cause a 2--4 fold increase in the rate of precursor incorporation and alter the pattern of label distribution after fractionation of the extracts by sedimentation through sucrose gradients or chromatography on MAK 1 columns. Using sterile senescing radish leaf discs, a stimulation of a2p-orthophosphate incorporation into various fractions of nucleic acid was observed as a result of kinetin treatment.