The effects of in vivo hydrocortisone on lymphocyte-mediated cytotoxicity

✍ Scribed by Paul Katz; Annette M. Zaytoun; James H. ; Lee Jr.

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 561 KB
• Volume: 27
• Category: Article
• ISSN: 0004-3591
• DOI: 10.1002/art.1780270112

✦ Synopsis

To examine the effects of in vivo hydrocortisone sodium succinate (HC) on natural killer (NK) cell and antibody-dependedt cellular cytotoxicity (ADCC), 11 normal adults received a single intrdvenous bolus of 400 mg hydrocortisone. Lymphocytes were tested for NK activity and ADCC usihg 51chromium (51Cr)-release and single cell cytotoxicity bssays against Molt-4 and sensitized RL O+ target cells; respectively. Four hours after injection, both NK and ADCC ac,ti%ty were transiently increased in the "Cr-release qystdh ( P < 0.05). At 4 hours, there was a twofold increase in the relative frequency of potentially eytotoxic iarget binding cells (P < 0.001) but the absolute number of these cells did not change (P < 0.1). However, the peribntage lysis of bound targets at 4 hours was not altered (P > 0.1).

These data suggest that: 1) lymphocytes participating in NK and ADCC reactions are refractory to the kinetic and functional effects of HC; 2) the increased lytic activity observed at 4 hours is due to a selective depletion of noncytotoxic cells from the circulation; and 3) .