Specific labeling is required for energy transfer measurements and to avoid artifacts in the use of fluorophores as reporter groups. Therefore, a method for specific modification by one of the most popular reagents for P-type ATPases (fluorescein 5-isothiocyanate) has been developed. Sulfhydryl reag
The effects of enzymes on the photobleaching of fluorescein and fluorescein isothiocyanate conjugates
โ Scribed by R.S. Davidson; J.E. Pratt
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 552 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1011-1344
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The objective of this study was to identify, through kinetic analysis of individual elementary reactions, the conditions under which a simple first-order photobleaching kinetic model is sufficient for quantitative fluorescence measurements, and those under which more complex photobleaching kinetics
The reversible formation of dithiourethanes from fluorescein isothiocyanate and mercaptoethanol or mercaptoethylamine has been studied. It is shown that it is possible to use the reaction as the basis for a spectrophotometric titration of a number of isothiocyanates with mercaptoethanol.