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The effects of cyclophosphamide on in vitro cytotoxic responses to a syngeneic tumour

✍ Scribed by Elizabeth J. Hancock; Douglas G. Kilburn

Publisher
Springer-Verlag
Year
1982
Tongue
English
Weight
514 KB
Volume
14
Category
Article
ISSN
0340-7004

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✦ Synopsis

We have studied the effects of treating DBA/2 mice with high doses of cyclophosphamide upon their subsequent ability to generate cytotoxic cells in vitro against syngeneic tumour antigens or alloantigens. High doses of cyclophosphamide (100-200 mg/kg body weight) eliminated the response to both antigens. The addition of normal DBA/2 thymocytes into these cultures restored the response to allogeneic cells but not to tumour cells. The anti-tumour response could be restored by the addition of interleukin 2 to the cultures. Treatment with high doses of cyclophosphamide decreased the number of anti-tumour cytotoxic cell precursors in the spleen, but did not affect the capacity of bulk cultures of spleen cells to produce interleukin2 when stimulated with the mitogen concanavalin A.

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## Abstract One or 4 weeks after grafting of a syngeneic sarcoma (T~2~) to C57BL/6 mice, lymph‐node cells (LNC) are cytotoxic __in vitro__ for the cells of this tumour. But after 2 weeks LNC are not cytotoxic or show a non‐significant activity. These second‐week LNC, added to cytotoxic lymphocytes