The effects of capsaicin on the metabolic activation of heterocyclic amines and on cytochrome P450 1A2 activity in hamster liver microsomes

✍ Scribed by Robert W. Teel; Zhuo Zhang; Huong Huynh; Solomon Hamilton

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 154 KB
• Volume: 11
• Category: Article
• ISSN: 0951-418X
• DOI: 10.1002/(sici)1099-1573(199708)11:5<358::aid-ptr102>3.0.co;2-s

✦ Synopsis

Capsaicin, the principal component of Capsicum fruits, strongly inhibited the in vitro mutagenic activity of five heterocyclic amines (HCAs) in Salmonella typhimurium strain TA98 by hamster liver microsomes. This correlated with the inhibition of microsome associated demethylation of methoxyresorufin (MROD) by capsaicin. MROD activity is mediated by cytochrome P450 1A2 and CYP450 1A2 mediates the metabolic activation of HCAs. Enzyme kinetics studies indicated a noncompetitive type of inhibition by capsaicin. Liver microsomes prepared from hamsters 6 h and 24 h after administration of a single oral dose of capsaicin in olive oil at either 2 mg/kg or 10 mg/kg body weight expressed a small but statistically significant inhibition of MROD activity compared with microsomes from control (untreated) animals. These results suggest that capsaicin can modulate the activity of cytochrome P450 1A2.