The effects of cadmium on succinate and NADH-linked substrate oxidations in rat hepatic mitochondria

✍ Scribed by I. Cameron; P. M. McNamee; A. Markham; R. M. Morgan; M. Wood

• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: English
• Weight: 565 KB
• Volume: 6
• Category: Article
• ISSN: 0260-437X
• DOI: 10.1002/jat.2550060505

✦ Synopsis

Low concentrations of cadmium (3.3-40 p ~) inhibited State 3 NADH-linked respiration in rat hepatic mitochondria, but failed to release oligomycin (1 pg) inhibited State 3 respiration, or to significantly change the State 4 rate. In the presence of succinate, 40 p~ cadmium inhibited State 3 respiration by 89%, while concentrations between 3.3 and 13.3 p~ stimulated State 4 respiration. Higher concentrations caused marked inhibition. In the presence of succinate, cadmium released oligomycin inhibited State 3 respiration. Cadmium (0.001-1.0 mM) did not stimulate mitochondrial ATPase activity or inhibit ferricyanide reduction, but stimulated NAD+ linked mitochondrial dehydrogenase activities and NADH oxidation. These results indicate that cadmium interacts with either the NADH dehydrogenase complex or other NADH-dependent enzymes and not solely by an uncoupling action.