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The Effects of Ca2+and Mg2+on the Major Gelatinase Activities Present in the Sea Urchin Embryo

✍ Scribed by John J. Robinson; Janice Mayne


Book ID
115581478
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
134 KB
Volume
243
Category
Article
ISSN
0006-291X

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Calcium–protein interactions in the extr
✍ Janice Mayne; John J. Robinson πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 329 KB πŸ‘ 1 views

We have purified and characterized a collagenase/gelatinase activity expressed during sea urchin embryonic development. The native molecular mass was determined to be 160 kDa, while gelatin substrate gel zymography revealed an active species of 41 kDa, suggesting that the native enzyme is a tetramer